Sites __exclusive__: Pgps3 Unique Restriction

Happy cloning! Have you found a rare unique site in PGPS3 that I missed? Or a tricky methylation issue? Drop a comment below or tag me on Twitter @PlantBiotechLab – let’s build a better restriction map together.

If you work with plant transformation, particularly in Arabidopsis thaliana or related species, you’ve likely encountered the PGPS3 vector. Derived from the pGreen series, PGPS3 is a compact, high-copy-number binary vector known for its small size (approximately 3.2 kb), making it ideal for golden gate cloning, site-directed mutagenesis, and efficient transformation into Agrobacterium tumefaciens . pgps3 unique restriction sites

Just remember: no SalI, no EcoRV, and always verify your map. When in doubt, a quick virtual digest takes 30 seconds and saves three days of failed ligations. Happy cloning